Recombinant Human Activin A

Synonym

Activin A,   INHBA

Source

Human Activin A is expressed from human 293 cells (HEK293).  It contains AA Gly 311 - Ser 426 .

Predicted N-terminus: Gly 311

Molecular Characterization

Activin A (Gly 311 - Ser 426):   GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

This protein carries no "tag".

The protein has a calculated MW of 13 kDa. The protein migrates as 14 kDa under reducing (R) condition and 27 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin

Less than 0.1 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 60% ACN, 0.056% TFA in water. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the Certificate of Analysis (will come with the product).

Cytokines: 

Formulation

Lyophilized from 0.22 μm filtered solution in 60% ACN, 0.056% TFA in water. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the Certificate of Analysis (will come with the product).

Human Activin A on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Immobilized Human Activin A, Tag Free at 10μg/mL (100 μL/well) can bind Human ACVR1B, Fc Tag with a linear range of 0.3-2.5 μg/mL (QC tested).

Immobilized Human Activin A, Tag Free 10μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag with a linear range of 0.08-0.625 μg/mL (QC tested).

The bio-activity of Activin A was determined by dose-dependent inhibition of the proliferation of MPC-11 cells. The EC50 for this effect is typically ≤ 9.5ng/ml (Routinely tested).

Background

Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. Activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle. Many other functions have been found to be exerted by activin, including roles in cell proliferation, differentiation, apoptosis, metabolism, homeostasis, immune response, wound repair, and endocrine function. Conversely inhibin down regulates FSH synthesis and inhibits FSH secretion. Activins are non-glycosylated homodimers or heterodimers of various β subunits (βA, βB, βC, and βE in mammals), while  Inhibins are heterodimers of a unique α subunit and one of the β subunits. Activin A is a widely expressed homodimer of two βA chains. The βA subunit can also heterodimerize with a βB or βC subunit to form Activin AB and Activin AC, respectively. The 14 kDa mature human βA chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat βA.

References

1. Chen YG., et al., 2006, Exp. Biol. Med. (Maywood) 231 (5): 534–44.
2. Sulyok S, et al., 2004, Mol. Cell. Endocrinol. 225 (1–2): 127–32.
3. van Zonneveld P, et al., 2003, Hum Reprod 18 (3): 495–501.
4. Harrison, C.A. et al., 2005, Trends Endocrinol. Metab. 16:73.
5. Thompson, T.B. et al., 2004, Mol. Cell. Endocrinol. 225:9.

Please contact us via info@humancellsbio.com if you have any question on this product.