Recombinant Human Flt-3 Ligand

Synonym

FLT3LG, FL, FLT3L, Flt3 ligand

Source

Human Flt-3 Ligand is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185

Predicted N-terminus: Thr 27

Molecular Characterization

Flt-3 Ligand (Thr 27 - Pro 185): TQDC SFQHSPISSD FAVKIRELSD YLLQDYPVTV ASNLQDEELC GGLWRLVLAQ RWMERLKTVA GSKMQGLLER VNTEIHFVTK CAFQPPPSCL RFVQTNISRL LQETSEQLVA LKPWITRQNF SRCLELQCQP DSSTLPPPWS PRPLEATAPT APQPP

This protein carries no "tag".

The protein has a calculated MW of 18.9 kDa. The protein migrates as 25-30 kDa on a SDS-PAGE gel under reducing (R) condition.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the Certificate of Analysis (will come with the product).

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

4-8°C for 12 months in lyophilized state;
‒70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human Flt-3 Ligand on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Background

FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalently linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.

References

Wodnar-Filipowicz A., 2003, News Physiol Sci., 18:247-51.
McClanahan T., et al., 1996, Blood, 88(9):3371-82.
Diener K.R. et al., 2008, Exp Hematol., 36(1):51-60.
Farag SS, Caligiuri MA, 2006, Blood Rev. 20(3):123-37.
Shortman, Ken, et al., 2006, Nature Reviews Immunology 7 (1): 19–30.

Please contact us via info@humancellsbio.com if you have any question on this product.