Human Cord Blood CD19+ B Cells
B cells, also known as B lymphocytes, express CD19 from very early stages, and the expression is only lost upon maturation into plasma cells. After maturing, the B cells migrate to secondary lymphatic organs and are subsequently activated directly by an antigen or through an antigen presenting cell (APS). At this point they either become memory B cells or plasma B cells. Each B cell gives rise to a single species of antibody.
Our Human Cord Blood CD19+ B cells are isolated from cord blood mononuclear cells by positive immunomagnetic selection.
All cord blood is collected in Citric Phosphate with Dextrose Buffer (CPD) from fully consented IRB approved donors who are tested negative for HIV, HBV and HCV.
Figure 1. Flow cytometric analysis showed that >95% cells are CD19 positive.