Human Peripheral Blood Mononuclear Cells (PBMCs) are blood cells with a single round nucleus. These cells include lymphocytes (T, B and NK cells), monocytes and dendritic cells. PBMCs are parts of the immune system which are critical to cell-mediated and humoral immunity.
Our Human PBMCs are isolated from normal healthy donor leukopaks collected in acid-citrate-dextrose formula A (ACDA) as an anticoagulant. All donors must be tested negative for HBV, HCV, HIV and Syphilis and are IRB consented.
Peripheral blood mononuclear cells (PBMCs) are purified by a density gradient centrifugation (Ficoll-Paque) and are available in fresh and frozen (cryopreserved) format. HumanCells' PBMC are isolated from Leuko Pak (Apheresis-derived) which are highly purified, containing a more pure population of mononuclear cells with higher viability when compared to buffy coat derived cells.
Cryopreserved PBMCs can be stored in Liquid Nitrogen vapor phase for long-term storage. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately, and can not be frozen again.